Mark Stenglein

Principal investigator
Google Scholar

I'm an Associate Professor in the Microbiology, Immunology and Pathology department at Colorado State University. My research aims to apply a variety of old and new techniques to better understand viruses. I am motivated to do this because viruses can (sometimes) cause disease but also because I find them endlessly fascinating.


A bioinformatics pipeline for a tick pathogen surveillance multiplex amplicon sequencing assay

Galbut Virus Infection Minimally Influences Drosophila melanogaster Fitness Traits in a Strain and Sex-Dependent Manner.

Divergent Serpentoviruses in Free-Ranging Invasive Pythons and Native Colubrids in Southern Florida, United States

Modeling cellular co-infection and reassortment of bluetongue virus in Culicoides midges

Rapid evolution of SARS-CoV-2 in domestic cats

The Case for Studying New Viruses of New Hosts.

Occurrence of Wheat Curl Mite and Mite-Vectored Viruses of Wheat in Colorado and Insights into the Wheat Virome

Bluetongue Research at a Crossroads. Modern Genomics Tools Can Pave the Way to New Insights

Seasonal Dynamics of Mosquito-Borne Viruses in the Southwestern Florida Everglades

Safety study of Rift Valley Fever human vaccine candidate (DDVax) in mosquitoes.

SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection

A Whole Virion Vaccine for COVID-19 Produced via a Novel Inactivation Method and Preliminary Demonstration of Efficacy in an Animal Challenge Model

Genomic characterization of 99 viruses from the bunyavirus families Nairoviridae, Peribunyaviridae, and Phenuiviridae, including 35 previously unsequenced viruses

In Vitro Reassortment between Endemic Bluetongue Viruses Features Global Shifts in Segment Frequencies and Preferred Segment Combinations

Serpentovirus (Nidovirus) and Orthoreovirus Coinfection in Captive Veiled Chameleons (Chamaeleo calyptratus) with Respiratory Disease

The Genetic Diversification of a Single Bluetongue Virus Strain Using an In Vitro Model of Alternating-Host Transmission

Cross-Species Transmission and Evolution of SIV Chimpanzee Progenitor Viruses Toward HIV-1 in Humanized Mice

Partitiviruses Infecting Drosophila melanogaster and Aedes aegypti Exhibit Efficient Biparental Vertical Transmission

A Novel Retrovirus (Gunnison's Prairie Dog Retrovirus) Associated With Thymic Lymphoma in Gunnison's Prairie Dogs in Colorado, USA

Ecological Dynamics Impacting Bluetongue Virus Transmission in North America

dsRNA-Seq Identification of Viral Infection by Purifying and Sequencing dsRNA

Longitudinal and Cross-Sectional Sampling of Serpentovirus (Nidovirus) Infection in Captive Snakes Reveals High Prevalence, Persistent Infection, and Increased Mortality in Pythons and Divergent Serpentovirus Infection in Boas and Colubrids

Strengthening the Interaction of the Virology Community with the International Committee on Taxonomy of Viruses (ICTV) by Linking Virus Names and Their Abbreviations to Virus Species

A reverse-transcription/RNase H based protocol for depletion of mosquito ribosomal RNA facilitates viral intrahost evolution analysis, transcriptomics and pathogen discovery.

Ebola virus, but not Marburg virus, replicates efficiently and without required adaptation in snake cells.

Respiratory disease in ball pythons (Python regius) experimentally infected with ball python nidovirus

Metagenomic Investigation of Idiopathic Meningoencephalomyelitis in Dogs

Modeling the evolution of SIV sooty mangabey progenitor virus towards HIV-2 using humanized mice

Differential disease susceptibility in experimentally reptarenavirus infected boa constrictors and ball pythons

Development and Characterization of Recombinant Virus Generated from a New World Zika Virus Infectious Clone

Detection and prevalence of boid inclusion body disease in collections of boas and pythons using immunological assays.

Possibility and Challenges of Conversion of Current Virus Species Names to Linnaean Binomials

West African Anopheles gambiae mosquitoes harbor a taxonomically diverse virome including new insect-specific flaviviruses, mononegaviruses, and totiviruses

Multiplexed Metagenomic Deep Sequencing To Analyze the Composition of High-Priority Pathogen Reagents

West Nile Virus Population Structure, Injury, and Interferon-Stimulated Gene Expression in the Brain From a Fatal Case of Encephalitis.

Age and prior blood feeding of Anopheles gambiae influences their susceptibility and gene expression patterns to ivermectin-containing blood meals.

Widespread Recombination, Reassortment, and Transmission of Unbalanced Compound Viral Genotypes in Natural Arenavirus Infections

Past, present, and future of arenavirus taxonomy

Genome Sequence of a Bornavirus Recovered from an African Garter Snake (Elapsoidea loveridgei).

Ball Python Nidovirus - a Candidate Etiologic Agent for Severe Respiratory Disease in Python regius.

Polar bear encephalitis - establishment of a comprehensive next-generation pathogen analysis pipeline for captive and free-living wildlife.

Structural characterization of the glycoprotein GP2 core domain from the CAS virus, a novel arenavirus-like species.

A novel endogenous betaretrovirus group characterized from polar bears (Ursus maritimus) and giant pandas (Ailuropoda melanoleuca).

Complete genome sequence of an astrovirus identified in a domestic rabbit (Oryctolagus cuniculus) with gastroenteritis.

Identification, characterization, and in vitro culture of highly divergent arenaviruses from boa constrictors and annulated tree boas - candidate etiological agents for snake inclusion body disease.

Virus identification in unknown tropical febrile illness cases using deep sequencing.

APOBEC3G contributes to HIV-1 variation through sublethal mutagenesis.

Quantitative profiling of the full APOBEC3 mRNA repertoire in lymphocytes and tissues - implications for HIV-1 restriction.

APOBEC3 proteins mediate the clearance of foreign DNA from human cells.

Two regions within the amino-terminal half of APOBEC3G cooperate to determine cytoplasmic localization.

The restriction of zoonotic PERV transmission by human APOBEC3G.

Evolutionarily conserved and non-conserved retrovirus restriction activities of artiodactyl APOBEC3F proteins.

APOBEC3B and APOBEC3F inhibit L1 retrotransposition by a DNA deamination-independent mechanism.


Award winning talks by Marylee and Lexi

There is no existing good evidence that 2019-nCoV originated in snakes.

Genomics of Disease in Wildlife Workshop, 2017

Shaun selected for a GAUSSI fellowship

New paper on mosquito viruses out

Rocky Mountain Virology Club

Goodbye, Joey!

Welcome, Jackie!

Lab hike in lieu of lab meeting

The lab is growing

Come join the fun!